Ambani is a surname, and there are many people with this surname. To answer the question accurately, we need to know which Ambani you're asking about.
For example, Mukesh Ambani, the chairman and managing director of Reliance Industries, is known to speak Gujarati, Hindi, and English.
Anil Ambani, the chairman of Reliance Group, also speaks Gujarati, Hindi, and English.
To determine which language a specific Ambani speaks, you need to provide more information about the individual you are referring to.