No, Harry did not lose his Parseltongue ability. He retained it throughout the series.
Parseltongue is a rare, magical ability to speak with snakes, inherited through the lineage of Salazar Slytherin. Harry, as a descendant of Slytherin, inherited this ability from his father, James Potter.
While Harry's Parseltongue ability was initially portrayed as a sign of his connection to Voldemort, it is later confirmed that he inherited this ability from his father, James Potter, who was also a Parselmouth.
Throughout the series, Harry demonstrates his ability to understand and speak with snakes on several occasions, including:
- The Chamber of Secrets: Harry discovers the Chamber of Secrets and speaks with the Basilisk, demonstrating his Parseltongue ability.
- The Goblet of Fire: Harry speaks with a snake in the graveyard, revealing his connection to Voldemort.
- The Deathly Hallows: Harry is shown speaking with a snake at the Burrow, further emphasizing his Parseltongue ability.
Therefore, Harry's Parseltongue ability remains with him throughout the series, and it is not lost at any point.