Absolutely! There's no rule that says a boy can't call a girl "bestie." The term "bestie" is a shortened version of "best friend" and is commonly used to express close friendship regardless of gender.
While some might associate "bestie" with a more feminine term, it's simply a friendly way to refer to your closest companion, regardless of their gender.
It's important to remember that language is constantly evolving, and terms like "bestie" are becoming increasingly gender-neutral.
So, if a boy calls a girl "bestie," it simply means he considers her a very close friend.