H<sub>2</sub>SO<sub>4</sub>, or sulfuric acid, is not typically used in ELISA (Enzyme-Linked Immunosorbent Assay) procedures. ELISA relies on enzymatic reactions and antibody-antigen interactions, and sulfuric acid is not involved in these processes.
It is possible that you may be thinking of a different reagent used in ELISA, such as:
- Washing buffer: This buffer typically contains a mild detergent like Tween 20 and a salt like PBS (Phosphate Buffered Saline). The purpose of the washing buffer is to remove unbound antibodies or antigens, ensuring only the specifically bound molecules remain.
- Substrate: This is a chemical that is converted by the enzyme linked to the antibody in the ELISA. The substrate conversion produces a detectable signal, like a color change.
If you could clarify the specific context where you encountered H<sub>2</sub>SO<sub>4</sub> in relation to ELISA, I can provide a more accurate answer.
